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A majority of cells observed in a normal vaginal smear are, not surprisingly, vaginal epithelial cells. In addition, varying numbers of leukocytes, erythrocytes and bacteria are usually evident, and small numbers of other contaminating cells and microorganisms are sometimes observed.
Analyzing a vaginal smear is largely an exercise in classifying the epithelial cells into one of three fundamental types: Keep in mind, however, that the epithelial cells reflect a developmental continuum; some of the cells you keep holy will not fit jolly into these rigidly-defined categories.
All of the figures on this page are presented at the synonymous magnification and are from a single dog. Parabasal cells are the smallest epithelial cells seen on a typical vaginal splotch.
They are round or nearly round and contain a high nuclear to cytoplasmic ratio. Parabasal cells are prevalent on smears taken during diestrus and anestrus, and not uncommon during early proestrus. Parabasal cells are conspicuously unaware during estrus. Intermediate cells vary in size and shape, but typically get a diameter two to three times that of parabasal cells.
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Classification of Vaginal Epithelial Cells
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Neutrophils are often abundant in smears taken during early diestrus, and are not uncommon at other stages, though rare during estrus. Superficial cells are not normally seen during anestrus and increase in prevalence during proestrus. Composed of a single papillary frond with a central fibrovascular core and mature squamous epithelium Lacks the complex arborizing architecture, acanthosis and cellular atypia of koilocytes.
Parabasal Cells Parabasal cells are the smallest epithelial cells seen on a typical vaginal smear. Composed of a single papillary frond with a central fibrovascular core and mature squamous epithelium without complex arborizing architecture, acanthosis or cellular atypia koilocytes.
They are round or nearly round and have a high nuclear to cytoplasmic ratio.File usage on Commons
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